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Comparison of Diagnostic Methods inCutaneous Leishmaniasis in Iraq

Received: 14 April 2013     Published: 2 May 2013
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Abstract

Sixty -four specimens collected passively from patients with cutaneous ulcers suggestive of leishmaniasis attending hospital and health centres in all parts of Iraq for diagnosis were included in the study. The present study was conducted from June to December 2012 in Al-Karamah Teaching Hospital in Wasit Province, Iraq. Dermal scrapings were analysed both by parasitological (examination of Giemsa-stained smears and in vitro cultivation) methods and by a genus-specific PCR detection. Microscopy revealed amastigotes in 38 samples (59.4%) and in vitro cultivation gave positive results in 19 cases (29.7%), whereas PCR detected Leishmania in 44 samples (68.75 %). There are two forms of Cutaneous leishmaniasis (CL) in Iraq are caused by two species of Leishmania . Of 44 well-documented cases in our study ; 28 (63.6 %) were caused byLeishmania major and 16 (36.4 %) by Leishmania tropica.

Published in American Journal of BioScience (Volume 1, Issue 1)
DOI 10.11648/j.ajbio.20130101.11
Page(s) 1-5
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

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Copyright © The Author(s), 2013. Published by Science Publishing Group

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Keywords

Cutaneous Leishmaniasis, PCR, Diagnosis

References
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  • APA Style

    Abdulsadah A. Rahi, Sundus Nsaif, Jaafar J. Hassoni, Magda A. Ali, Hassan A. Hamza. (2013). Comparison of Diagnostic Methods inCutaneous Leishmaniasis in Iraq. American Journal of BioScience, 1(1), 1-5. https://doi.org/10.11648/j.ajbio.20130101.11

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    ACS Style

    Abdulsadah A. Rahi; Sundus Nsaif; Jaafar J. Hassoni; Magda A. Ali; Hassan A. Hamza. Comparison of Diagnostic Methods inCutaneous Leishmaniasis in Iraq. Am. J. BioScience 2013, 1(1), 1-5. doi: 10.11648/j.ajbio.20130101.11

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    AMA Style

    Abdulsadah A. Rahi, Sundus Nsaif, Jaafar J. Hassoni, Magda A. Ali, Hassan A. Hamza. Comparison of Diagnostic Methods inCutaneous Leishmaniasis in Iraq. Am J BioScience. 2013;1(1):1-5. doi: 10.11648/j.ajbio.20130101.11

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  • @article{10.11648/j.ajbio.20130101.11,
      author = {Abdulsadah A. Rahi and Sundus Nsaif and Jaafar J. Hassoni and Magda A. Ali and Hassan A. Hamza},
      title = {Comparison of Diagnostic Methods inCutaneous Leishmaniasis in Iraq},
      journal = {American Journal of BioScience},
      volume = {1},
      number = {1},
      pages = {1-5},
      doi = {10.11648/j.ajbio.20130101.11},
      url = {https://doi.org/10.11648/j.ajbio.20130101.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20130101.11},
      abstract = {Sixty -four specimens collected passively from patients with cutaneous ulcers suggestive of leishmaniasis attending hospital and health centres in all parts of Iraq for diagnosis were included in the study. The present study was conducted from June to December 2012 in Al-Karamah Teaching Hospital in Wasit Province, Iraq. Dermal scrapings were analysed both by parasitological (examination of Giemsa-stained smears and in vitro cultivation) methods and by a genus-specific PCR detection. Microscopy revealed amastigotes in 38 samples (59.4%) and in vitro cultivation gave positive results in 19 cases (29.7%), whereas PCR detected Leishmania in 44 samples (68.75 %). There are two forms of Cutaneous leishmaniasis (CL) in Iraq are caused by two species of Leishmania . Of 44 well-documented cases in our study ; 28 (63.6 %) were caused byLeishmania major and 16 (36.4 %) by Leishmania tropica.},
     year = {2013}
    }
    

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  • TY  - JOUR
    T1  - Comparison of Diagnostic Methods inCutaneous Leishmaniasis in Iraq
    AU  - Abdulsadah A. Rahi
    AU  - Sundus Nsaif
    AU  - Jaafar J. Hassoni
    AU  - Magda A. Ali
    AU  - Hassan A. Hamza
    Y1  - 2013/05/02
    PY  - 2013
    N1  - https://doi.org/10.11648/j.ajbio.20130101.11
    DO  - 10.11648/j.ajbio.20130101.11
    T2  - American Journal of BioScience
    JF  - American Journal of BioScience
    JO  - American Journal of BioScience
    SP  - 1
    EP  - 5
    PB  - Science Publishing Group
    SN  - 2330-0167
    UR  - https://doi.org/10.11648/j.ajbio.20130101.11
    AB  - Sixty -four specimens collected passively from patients with cutaneous ulcers suggestive of leishmaniasis attending hospital and health centres in all parts of Iraq for diagnosis were included in the study. The present study was conducted from June to December 2012 in Al-Karamah Teaching Hospital in Wasit Province, Iraq. Dermal scrapings were analysed both by parasitological (examination of Giemsa-stained smears and in vitro cultivation) methods and by a genus-specific PCR detection. Microscopy revealed amastigotes in 38 samples (59.4%) and in vitro cultivation gave positive results in 19 cases (29.7%), whereas PCR detected Leishmania in 44 samples (68.75 %). There are two forms of Cutaneous leishmaniasis (CL) in Iraq are caused by two species of Leishmania . Of 44 well-documented cases in our study ; 28 (63.6 %) were caused byLeishmania major and 16 (36.4 %) by Leishmania tropica.
    VL  - 1
    IS  - 1
    ER  - 

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Author Information
  • College of Science/Wasit University, Iraq

  • College of Medicine/Kufa University, Iraq

  • College of Medicine/Wasit University, Iraq

  • College of Medicine/Wasit University, Iraq, TUMS-IC

  • Al-Sadir Hospital/Ministry of Health, Iraq

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