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Genotyping of Erythrocyte Binding Antigen 175 KD (eba-175) Alleles of Plasmodium Falciparum-Malaria Using Nested-PCR in South-East of Iran

Received: 12 March 2014     Accepted: 8 April 2014     Published: 10 April 2014
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Abstract

The Erythrocyte Binding Antigen-175 (EBA-175) on Plasmodium falciparum (P.falciparum) merozoites mediates sialic acid dependent binding to glycophorin a on host erythrocytes and, therefore, plays a crucial role in cell invasion. Dimorphic allele segments have been found in its encoding gene with in FCR-3 strains (F-segment) and CAMP strains (C-segment). This study was designed to determine the distribution of EBA-175 alleles of P.falciparum in the South-East of Iran. Nested Polymerase Chain Reaction method used with specific primers, which improves the two fragments of the EBA-175 gene. Ninety-four microscopically positive blood samples were collected from the infected P.falciparum malaria patients from four different districts. The 88 out of 94 confirmed P.falciparum samples were successfully scored for EBA-175. The allelic genotyping exhibited CAMP strains (714 bp) and FCR-3 strains (795 bp) in 31(32.97%) and 49(52.12%) cases, respectively and 8 cases (8.51%) showed mixed allelic CAMP/FCR-3 infections. The two fragments of dimorphic EBA-175 gene were observed and the FCR-3 allele was more prevalent in South-East of Iran. This distributional pattern should be considered in designing to control P. falciparum malaria in the region.

Published in American Journal of BioScience (Volume 2, Issue 2)
DOI 10.11648/j.ajbio.20140202.16
Page(s) 56-59
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Plasmodium falciparum, Erythrocyte Binding Antigen-175, South-East of Iran

References
[1] NH. Tolia, EJ. Enemark, BK. Sim, L. Joshua-Tor, "Structural basis for the EBA-175 erythrocyte invasion pathway of the malaria parasite Plasmodium falciparum", Cell. 122(2): p. 183-93,2005.
[2] DS. Perce-da-Silva, DM. Banic, JC. Lima-Junior, F. Santos, CT. Daniel-Ribeiro, J. de Oliveira-Ferreira, et al, "Evaluation of allelic forms of the erythrocyte binding antigen 175 (EBA-175) in Plasmodium falciparum field isolates from Brazilian endemic area", Malar J, 10: p. 146, 2011.
[3] SA. Al-Harthi, "Malaria drug resistant: current situation with reference to Saudi Arabia (review)", J Egypt Soc Parasitol, 41(3): p. 553-64, 2011.
[4] A. Salvador, RM. Hernandez, JL. Pedraz, M. Igartua, "Plasmodium falciparum malaria vaccines: current status, pitfalls and future directions", Expert Rev Vaccines, 11(9): p. 1071-86, 2012.
[5] I. Soulama, JD. Bigoga, M. Ndiaye, EC. Bougouma, J. Quagraine, PN. Casimiro, et al, "Genetic diversity of polymorphic vaccine candidate antigens (apical membrane antigen-1, merozoite surface protein-3, and erythrocyte binding antigen-175) in Plasmodium falciparum isolates from western and central Africa", Am J Trop Med Hyg, 84(2): p. 276-84, 2011.
[6] I. Soulama, EC. Bougouma, A. Diarra, I. Nebie, SB. Sirima, "Low-high season variation in Plasmodium falciparum erythrocyte binding antigen 175 (eba-175) allelic forms in malaria endemic area of Burkina Faso", Trop Med Int Health, 15(1): p. 51-9, 2010.
[7] DS. Peterson, LH. Miller, TE. Wellems, "Isolation of multiple sequences from the Plasmodium falciparum genome that encode conserved domains homologous to those in erythrocyte-binding proteins", Proc Natl Acad Sci U S A, 92(15): p. 7100-4, 1995.
[8] DC. Mayer, O. Kaneko, DE. Hudson-Taylor, ME. Reid, LH. Miller, "Characterization of a Plasmodium falciparum erythrocyte-binding protein paralogous to EBA-175", Proc Natl Acad Sci U S A, 98(9): p. 5222-7, 2001.
[9] BK. Sim, PA. Orlandi, JD. Haynes, FW. Klotz, JM. Carter, D. Camus, et al, "Primary structure of the 175K Plasmodium falciparum erythrocyte binding antigen and identification of a peptide which elicits antibodies that inhibit malaria merozoite invasion", J Cell Biol, 111(5 Pt 1): p. 1877-84, 1990.
[10] A. Ebrahimzadeh, M. Polshekan, M. Qureshi, I. Sharifi, "Reliable DNA Extraction on Historical Malaria Negative Smears Conducted to Nested PCR in South-East of Iran", Biotechnology, 5(3): p. 5, 2006.
[11] A. Ebrahimzadeh, A. Fazaeli, "The Risk of Re-Emergence of Plasmodium malariae in South-East of Iran as Detected by Nested Polymerase Chain Reaction", Asian Journal of Epidemiology, 1(2): p. 6, 2008.
[12] A. Ebrahimzadeh, B. Fouladi, A. Fazaeli, "High rate of detection of mixed infections of Plasmodium vivax and Plasmodium falciparum in South-East of Iran, using nested PCR", Parasitol Int, 56(1): p. 61-4, 2007.
[13] A. Heidari, H. Keshavarz, S. Dittrich, T. Jelinek, "Allelic Dimorphism of the Plasmodium falciparum ErythrocyteBinding Antigen-175 (EBA-175) Gene in the Southeast of Iran", Iranian J Parasitol, 4(2): p. 17-22, 2009.
[14] S. Dittrich, B. Schwobel, S. Jordan, V. Vanisaveth, P. Rattanaxay, EM. Christophel, et al, "Distribution of the two forms of Plasmodium falciparum erythrocyte binding antigen-175 (eba-175) gene in Lao PDR", J. Malar, 2: p. 23, 2003.
[15] FS. Toure, C. Bisseye, E. Mavoungou, "Imbalanced distribution of Plasmodium falciparum EBA-175 genotypes related to clinical status in children from Bakoumba, Gabon", Clin Med Res, 4(1): p. 7-11, 2006.
[16] PC. Jakob, PM. Frank, I. Möhl, S. Dittrich, D. Ekkehardt, NO. Rowland, et al, "Allelic dimorphism of the erythocyte binding antigen-175 (eba-175) gene of Plasmodium falciparum and severe malaria: Significant association of the C-segment with fatal outcome in Ghanaian children", Malar J, 3(11): p. 1-6, 2004.
[17] R. Binks, J. Baum, A. Oduola, D. Arnot, H. Babiker, P. Kremsner, et al, "Population genetic analysis of the Plasmodium falciparum erythrocyte binding antigene-175 (eba-175) gene", Mol Biochem Parasitol, 114(1): p. 63-70, 2001.
[18] A. Esmailli, M. Nateghpour, M. Asmar, M. Razavi, H. Kanbara, H. Uemura, et al, "Detection of K76 T mutation pfcrt gene as an applicable genetic marker for prediction of choloroquine resistant falciparum malaria in isolates from an endemic district of Iran", Iranian J Parasitol, 3(2): p. 48-56, 2008.
[19] MU. Ferreira, M. da Silva Nunes, G. Wunderlich, "Antigenic diversity and immune evasion by malaria parasites", Clin Diagn Lab Immunol, 11(6): p. 987-95, 2004.
[20] A. Heidari, S. Dittrich, T. Jelinek, A. Kheirandish, K. Banihashemi, H. Keshavarz, "Genotypes and Invivo Resistance of Plasmodium falciparum Isolates in an Endemic Region of Iran", Parasitol Res, 100: p. 589-592, 2007.
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  • APA Style

    Ebrahimzadeh Adel, Jamshidi Ali. (2014). Genotyping of Erythrocyte Binding Antigen 175 KD (eba-175) Alleles of Plasmodium Falciparum-Malaria Using Nested-PCR in South-East of Iran. American Journal of BioScience, 2(2), 56-59. https://doi.org/10.11648/j.ajbio.20140202.16

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    ACS Style

    Ebrahimzadeh Adel; Jamshidi Ali. Genotyping of Erythrocyte Binding Antigen 175 KD (eba-175) Alleles of Plasmodium Falciparum-Malaria Using Nested-PCR in South-East of Iran. Am. J. BioScience 2014, 2(2), 56-59. doi: 10.11648/j.ajbio.20140202.16

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    AMA Style

    Ebrahimzadeh Adel, Jamshidi Ali. Genotyping of Erythrocyte Binding Antigen 175 KD (eba-175) Alleles of Plasmodium Falciparum-Malaria Using Nested-PCR in South-East of Iran. Am J BioScience. 2014;2(2):56-59. doi: 10.11648/j.ajbio.20140202.16

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  • @article{10.11648/j.ajbio.20140202.16,
      author = {Ebrahimzadeh Adel and Jamshidi Ali},
      title = {Genotyping of Erythrocyte Binding Antigen 175 KD (eba-175) Alleles of Plasmodium Falciparum-Malaria Using Nested-PCR in South-East of Iran},
      journal = {American Journal of BioScience},
      volume = {2},
      number = {2},
      pages = {56-59},
      doi = {10.11648/j.ajbio.20140202.16},
      url = {https://doi.org/10.11648/j.ajbio.20140202.16},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20140202.16},
      abstract = {The Erythrocyte Binding Antigen-175 (EBA-175) on Plasmodium falciparum (P.falciparum) merozoites mediates sialic acid dependent binding to glycophorin a on host erythrocytes and, therefore, plays a crucial role in cell invasion. Dimorphic allele segments have been found in its encoding gene with in FCR-3 strains (F-segment) and CAMP strains (C-segment). This study was designed to determine the distribution of EBA-175 alleles of P.falciparum in the South-East of Iran. Nested Polymerase Chain Reaction method used with specific primers, which improves the two fragments of the EBA-175 gene. Ninety-four microscopically positive blood samples were collected from the infected P.falciparum malaria patients from four different districts. The 88 out of 94 confirmed P.falciparum samples were successfully scored for EBA-175. The allelic genotyping exhibited CAMP strains (714 bp) and FCR-3 strains (795 bp) in 31(32.97%) and 49(52.12%) cases, respectively and 8 cases (8.51%) showed mixed allelic CAMP/FCR-3 infections. The two fragments of dimorphic EBA-175 gene were observed and the FCR-3 allele was more prevalent in South-East of Iran. This distributional pattern should be considered in designing to control P. falciparum malaria in the region.},
     year = {2014}
    }
    

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  • TY  - JOUR
    T1  - Genotyping of Erythrocyte Binding Antigen 175 KD (eba-175) Alleles of Plasmodium Falciparum-Malaria Using Nested-PCR in South-East of Iran
    AU  - Ebrahimzadeh Adel
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    Y1  - 2014/04/10
    PY  - 2014
    N1  - https://doi.org/10.11648/j.ajbio.20140202.16
    DO  - 10.11648/j.ajbio.20140202.16
    T2  - American Journal of BioScience
    JF  - American Journal of BioScience
    JO  - American Journal of BioScience
    SP  - 56
    EP  - 59
    PB  - Science Publishing Group
    SN  - 2330-0167
    UR  - https://doi.org/10.11648/j.ajbio.20140202.16
    AB  - The Erythrocyte Binding Antigen-175 (EBA-175) on Plasmodium falciparum (P.falciparum) merozoites mediates sialic acid dependent binding to glycophorin a on host erythrocytes and, therefore, plays a crucial role in cell invasion. Dimorphic allele segments have been found in its encoding gene with in FCR-3 strains (F-segment) and CAMP strains (C-segment). This study was designed to determine the distribution of EBA-175 alleles of P.falciparum in the South-East of Iran. Nested Polymerase Chain Reaction method used with specific primers, which improves the two fragments of the EBA-175 gene. Ninety-four microscopically positive blood samples were collected from the infected P.falciparum malaria patients from four different districts. The 88 out of 94 confirmed P.falciparum samples were successfully scored for EBA-175. The allelic genotyping exhibited CAMP strains (714 bp) and FCR-3 strains (795 bp) in 31(32.97%) and 49(52.12%) cases, respectively and 8 cases (8.51%) showed mixed allelic CAMP/FCR-3 infections. The two fragments of dimorphic EBA-175 gene were observed and the FCR-3 allele was more prevalent in South-East of Iran. This distributional pattern should be considered in designing to control P. falciparum malaria in the region.
    VL  - 2
    IS  - 2
    ER  - 

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Author Information
  • Department of Medical Parasitology and Mycology and Membership of Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, Iran

  • Department of Medical Parasitology and Mycology, Zahedan University of Medical Sciences, Zahedan, Iran

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