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Isolation and Characterization of Water Soluble Polysaccharides from Isaria cicadae

Received: 13 September 2018     Accepted: 11 January 2019     Published: 26 February 2019
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Abstract

Isaria cicadae Miquel is an important medicinal food fungus. The crude polysaccharides from I. cicadae were shown to significantly extend the lifespan of fruit flies in our previous study. To understand the underlying mechanism, the composition and structural characterization of polysaccharides from I. cicadae were determined. A water-soluble crude polysaccharide, obtained from a cultured Isaria cicadae by boiling-water extraction and ethanol precipitation, was fractionated by DEAE-Sepharose column chromatography. The isolate gave four polysaccharide fractions termed B-I-1, B-I-2, B-II-1 and B-II-2. The average ratio of Glu: Man: Gla in B-I-2 was 1.28:1.25:1, B-II-1 was 16.08:1:1.95, and B-II-2, was 1:2.43:2.61. B-I-1 was composed of Ara, Xyl and Glu in the ratio of 1:2.36:33.14. GC and FTIR spectra analysis combined with NMR spectroscopic analysis gave preliminary structures of the four polysaccharide fractions, which may provide an elucidation on the action mechanisms of - polysaccharide on fruit flies proliferation.

Published in American Journal of BioScience (Volume 6, Issue 6)
DOI 10.11648/j.ajbio.20180606.11
Page(s) 57-64
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Isaria cicadae, Polysaccharides, GC, FTIR Spectra, NMR Spectra

References
[1] Sung, G. H., Hywel-Jones, N. L., Sung, J. M., Luangsa-ard, J. J., Shrestha, B., & Spatafora, J. W. (2007). Phylogenetic classification of Cordyceps and the clavicipitaceous fungi. Studies in mycology, 57, 5-59.
[2] Kuo, Y. C., Weng, S. C., Chou, C. J., Chang, T. T., & Tsai, W. J. (2003). Activation and proliferation signals in primary human t lymphocytes inhibited by ergosterol peroxide isolated from cordyceps cicadae. British Journal of Pharmacology, 140, 895–906.
[3] Kim, H. S., Kim, J. Y., Ryu, H. S., Shin, B. R., Kang, J. S., & Kim, H. M., et al. (2011). Phenotypic and functional maturation of dendritic cells induced by polysaccharide isolated from Paecilomyces cicadae. Journal of Medicinal Food, 14, 847–856.
[4] Xu Z, Song Z, Yan X, Li W, Zhao W, Ma H, Du J, Li S, Zhang D. (2018). Two heteropolysaccharides from Isaria cicadae Miquel differ in composition and potentially immunomodulatory activity. International Journal of Biological Macromolecules, 117:610-616.
[5] Wang, H., Zhang, J., Sit, W. H., Lee, C. Y. J., & Wan, M. F. (2014). Cordyceps cicadae induces G2/M cell cycle arrest in MHCC97H human hepatocellular carcinoma cells: a proteomic study. Chinese Medicine, 9, 15.
[6] Wang, J., Zhang, D. M., Jia, J. F., Peng, Q. L., Tian, H. Y., & Wang, L., et al. (2014). Cyclodepsipeptides from the ascocarps and insect-body portions of fungus Cordyceps cicadae. Fitoterapia, 97, 23–27.
[7] Lu CL, Lin HI, Chen BF, Jow GM. (2016). Beauvericin-induced cell apoptosis through the mitogen-activated protein kinase pathway in human nonsmall cell lung cancer A549 cells. Journal of Toxicological Sciences, 41 (3):429.
[8] Wang D, Wang J, Wang D, Yu X, Olatunji OJ, Ouyang Z, Wei Y. (2017). Neuroprotective effects of butanol fraction of Cordyceps cicadae on glutamate-induced damage in PC12 cells involving oxidative toxicity. Chemistry & Biodiversity, 15 (1): e1700385.
[9] Zhu, R., Chen, Y. P., Deng, Y. Y., Zheng, R., Zhong, Y. F., & Wang, L., et al. (2011). Cordyceps cicadae extracts ameliorate renal malfunction in a remnant kidney model. Journal of Zhejiang University B, 12, 1024-1033.
[10] Yu, S. J., Bao, J. Y., & Fan, M. Z. (2014). Extraction technology and biological activities of crude polysaccharide from synnemata of Cordyceps cicadae culture. Journal of Fungal Research, 12, 100-106.
[11] Galonic, D. P., Gin, D. Y. (2007). Chemical glycosylation in the synthesis of glycoconjugate antitumour vaccines. Nature, 446, 1000–1007.
[12] Yu, R. M., Yin, Y., Yang, W., Ma, W., Yang, L., & Chen, X., et al. (2009). Structural elucidation and biological activity of a novel polysaccharide by alkaline extraction from cultured Cordyceps militaris. Carbohydrate Polymers, 75, 166–171.
[13] Ukai, S., Matsuura, S., Hara, C., Kiho, T., & Hirose, K. (1982). Structure of a new galactomannan from the ascocarps of Cordyceps cicadae shing. Carbohydrate Research, 101, 109–116.
[14] Kiho, T., Miyamoto, I., Nagai, K., Ukai, S., & Hara, C. (1988). Minor, protein-containing galactomannans from the insect-body portion of the fungal preparation chán huā (Cordyceps cicadae). Carbohydrate Research, 181, 207–215.
[15] Zha, S. H., Zhao, Q. S., Chen, J. J., Wang, L. W., Zhang, G. F., Zhang, H., et al. (2014). Extraction, purification and antioxidant activities of the polysaccharides from maca (Lepidium meyenii). Carbohydrate Polymers, 111, 584-587.
[16] De Paula Regina, C. M., Heatley, F., & Budd, P. M. (1998). Characterization of Anacardiumoccidentale exudate polysaccharide. Polymer International, 45, 27–35.
[17] Heyn, A. N. J. (1974). The infrared absorption spectrum of dextran and its bound water. Biopolymers, 13, 475–506.
[18] Galat, A. (1980). Study of the Raman scattering and infrared absorption spectra of branched polysaccharides. Acta Biochimica Polonica, 27, 135–142.
[19] Kacurakova, M., Capek, P., Sasinkova, V., Wellner, N., & Ebringerova, A. (2000). FT-IR study of plant cell wall model compounds: Pectic polysaccharides andhemicelluloses. Carbohydrate Polymers, 43, 195–203.
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    Changsheng Sun, Shijun Yu, Jiayuan Bao, Meizhen Fan. (2019). Isolation and Characterization of Water Soluble Polysaccharides from Isaria cicadae. American Journal of BioScience, 6(6), 57-64. https://doi.org/10.11648/j.ajbio.20180606.11

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    ACS Style

    Changsheng Sun; Shijun Yu; Jiayuan Bao; Meizhen Fan. Isolation and Characterization of Water Soluble Polysaccharides from Isaria cicadae. Am. J. BioScience 2019, 6(6), 57-64. doi: 10.11648/j.ajbio.20180606.11

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    AMA Style

    Changsheng Sun, Shijun Yu, Jiayuan Bao, Meizhen Fan. Isolation and Characterization of Water Soluble Polysaccharides from Isaria cicadae. Am J BioScience. 2019;6(6):57-64. doi: 10.11648/j.ajbio.20180606.11

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  • @article{10.11648/j.ajbio.20180606.11,
      author = {Changsheng Sun and Shijun Yu and Jiayuan Bao and Meizhen Fan},
      title = {Isolation and Characterization of Water Soluble Polysaccharides from Isaria cicadae},
      journal = {American Journal of BioScience},
      volume = {6},
      number = {6},
      pages = {57-64},
      doi = {10.11648/j.ajbio.20180606.11},
      url = {https://doi.org/10.11648/j.ajbio.20180606.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20180606.11},
      abstract = {Isaria cicadae Miquel is an important medicinal food fungus. The crude polysaccharides from I. cicadae were shown to significantly extend the lifespan of fruit flies in our previous study. To understand the underlying mechanism, the composition and structural characterization of polysaccharides from I. cicadae were determined. A water-soluble crude polysaccharide, obtained from a cultured Isaria cicadae by boiling-water extraction and ethanol precipitation, was fractionated by DEAE-Sepharose column chromatography. The isolate gave four polysaccharide fractions termed B-I-1, B-I-2, B-II-1 and B-II-2. The average ratio of Glu: Man: Gla in B-I-2 was 1.28:1.25:1, B-II-1 was 16.08:1:1.95, and B-II-2, was 1:2.43:2.61. B-I-1 was composed of Ara, Xyl and Glu in the ratio of 1:2.36:33.14. GC and FTIR spectra analysis combined with NMR spectroscopic analysis gave preliminary structures of the four polysaccharide fractions, which may provide an elucidation on the action mechanisms of - polysaccharide on fruit flies proliferation.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - Isolation and Characterization of Water Soluble Polysaccharides from Isaria cicadae
    AU  - Changsheng Sun
    AU  - Shijun Yu
    AU  - Jiayuan Bao
    AU  - Meizhen Fan
    Y1  - 2019/02/26
    PY  - 2019
    N1  - https://doi.org/10.11648/j.ajbio.20180606.11
    DO  - 10.11648/j.ajbio.20180606.11
    T2  - American Journal of BioScience
    JF  - American Journal of BioScience
    JO  - American Journal of BioScience
    SP  - 57
    EP  - 64
    PB  - Science Publishing Group
    SN  - 2330-0167
    UR  - https://doi.org/10.11648/j.ajbio.20180606.11
    AB  - Isaria cicadae Miquel is an important medicinal food fungus. The crude polysaccharides from I. cicadae were shown to significantly extend the lifespan of fruit flies in our previous study. To understand the underlying mechanism, the composition and structural characterization of polysaccharides from I. cicadae were determined. A water-soluble crude polysaccharide, obtained from a cultured Isaria cicadae by boiling-water extraction and ethanol precipitation, was fractionated by DEAE-Sepharose column chromatography. The isolate gave four polysaccharide fractions termed B-I-1, B-I-2, B-II-1 and B-II-2. The average ratio of Glu: Man: Gla in B-I-2 was 1.28:1.25:1, B-II-1 was 16.08:1:1.95, and B-II-2, was 1:2.43:2.61. B-I-1 was composed of Ara, Xyl and Glu in the ratio of 1:2.36:33.14. GC and FTIR spectra analysis combined with NMR spectroscopic analysis gave preliminary structures of the four polysaccharide fractions, which may provide an elucidation on the action mechanisms of - polysaccharide on fruit flies proliferation.
    VL  - 6
    IS  - 6
    ER  - 

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Author Information
  • Zhejiang BioAsia Bio-Pharmaceutical Co., Ltd, Pinghu, China

  • Anhui Provincial Key Laboratory of Microbial Control, Anhui Agriculture University, Hefei, China

  • Anhui Provincial Key Laboratory of Microbial Control, Anhui Agriculture University, Hefei, China

  • Zhejiang BioAsia Bio-Pharmaceutical Co., Ltd, Pinghu, China

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