| Peer-Reviewed

Identification Phytochemicals and Antibacterial Activity of Coir Pith Part of Cassava Root

Received: 11 August 2022     Accepted: 20 October 2022     Published: 30 October 2022
Views:       Downloads:
Abstract

The purpose of the present study was to identify phytochemicals and anti-microbial activity of the coir pith part extract of cassava root. Preliminary Phytochemical screening was carried out for all the three extracts: n-hexane, methanol and ethanol as per the standard methods. Antibacterial activity of methanol crude extract of the coir pith part (M. esculenta) was performed and it was carried out by using agar well diffusion method. The preliminary phytochemical screening shows alkaloids and flavonoids present only on methanol crude extract and tannins, saponins, flavonoids, glycosides steroids, terpenoids and phenols are available on ethanol, methanol and n- hexane crude extracts. The zone of inhibition of methanolic extract of M. esculenta is 13.67 ± 0.58 mm, 16.67 ± 0.57mm and 18.33 ± 0.58mm at concentration of 25mg/ml, 50mg/ml and 100mg/ml in P. aeruginosa, respectively, and 12.33 ± 1.53mm, 13 ± 1.00mm and 15.33 ± 0.58mm at concentration of 25mg/ml, 50mg/ml and 100mg/ml in S. aureus, respectively. Moreover, the extract also showed moderate antibacterial activity at all concentrations in the range between 12 mm to 18mm on both tested organisms. So, the present study concludes that the coir pith extract anti-nutrients because of the presence of tannins, saponins, steroids and phenols.

Published in American Journal of BioScience (Volume 10, Issue 5)
DOI 10.11648/j.ajbio.20221005.14
Page(s) 180-185
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Anti-Bacterial, Cassava Root, Phytochemical

References
[1] Ani, A. I., Atangwho, I. J., Agiang M. A., and Y. E. Alozie. 2012. Biochemical effects of some cassava (Manihot esculenta, Crantz) traditional Nigerian diets in experimental diabetic rat. International Journal Biochemistry Research, 2 (2): 70-77.
[2] Atehnkng, J., and Adetimirin, V. O. 2006. Exploring the African cassava (Manihot esculenta Crantz) germplasm for somatic embryogenic competence: African J. Biotech., 5 (14): 1324-1329.
[3] Awouafack, MD., Mc Graw, LJ., Gottfried, S., Mbouangouere, R., Tane, P., Spiteller, M., and Eloff, JN. 2013. Antimicrobial activity and cytotoxicity of the ethanol extract, fractions and eight compounds isolated from Eriosemarobustum (Fabaceae): BMC Complement Altern Med. 13: 1.
[4] Dorman, H. J. D. and Stanley, G. 2000. Antimicrobial agents from plants. J. applied biology, Dol: 10.1046/j.1365-2672.2000.00969.x.
[5] Espina, L., Somolinos, M., Loran, S., Conchello, P., Garcia, D., and Pagan, R. 2011. Chemical composition of commercial citrus fruit essential oils and evaluation of their antimicrobial activity acting alone or in combined processes. Food control, 22 (6), 896-902.
[6] Fernebro J, 2011. Fighting bacterial infections Future treatment options: Drug Resist Update, 14: 125–39.
[7] Ganesh, S., and Vennil, J. J. 2011. Phytochemical Analysis of Acanthus ilicifolius and Avicennia officinalis by GC-MS. Res. J. Phytochem, 5: 60-65.
[8] Idris, S., Rosnah, S., Nor, M. Z. M., mokhtar, M. N. and Abdul Gani, S. S. 2019. Physicochemical composition of different parts of cassava (Manihot esculenta Crantz) plant: Halal products Research institute, 78–84, DOI: //doi.org/10.26656/fr.2019.4(s1).
[9] Jigna, P., and Sumitra, CV. 2007. In-vitro antimicrobial activity and phytochemical analysis of some Indian Medicinal plants. Turk J Biol. 31: 53-58.
[10] Lowy, F., 2003. Antimicrobial resistance: the example of Staphylococcus aureus: J. Clin Invest, 111: 1265–73.
[11] Musa, M. S., Abdelrasool, F. E., Elsheikh, E. A., Ahmed, L. A. M. N., Mahmoud, A. L. E., and Yagi, S. M. 2011. Ethnobotanical study of medicinal plants in the Blue Nile State, South-eastern Sudan. J. Medicinal Plants Research, 5 (17): 4287–4297.
[12] Ojelere, O. O., 2016. Phytochemicals, proximate, mineral element composition and antimicrobial activity of some selected medicinal plant seeds: DOI: 10.13140/RG.2.1.2160.2808.
[13] Okamoto, Y., Suzuki, A., Ueda, ito, C., itoigoawa, H., N, K, 2006. Anti-estrogen activity of prenylated isoflavones from milettia pachycarpa. J. Health. Sci. 52, 186-191.
[14] Okeke, B. C., Jacques, R. J., Bento, F. M., Peralba, M. C., and Camargo, F. A. 2007. Characterization of a polycyclic aromatic hydrocarbon-degrading microbial consortium from a petrochemical sludge land farming site. Bioremediation Journal, 11 (1), 1-11.
[15] Perez, C., Paul, M., Bazerque, P. 1990. An antibiotic assay by the agar-well diffusion method. Acta Biol Med Exp. 15: 113-115.
[16] Prashant, T., Bimlesh, K., Mandeep, K., Gurpreet, K., Haleen, K. 2011. Phytochemical screening and extraction. Inter. pharma. sci. 1: 98-104.
[17] Savithramma, N., Venkateswarlu, P., Suhrulatha, D., Basha, SKM., and Venkataramanadevi, CH. 2011. Studies of Boswelli aovalifoliolata Bal. and Herny - An endemic and endangered medicinal plant: The Biosc. 5: 359-362.
[18] Temesgen, Z., Bakalo, B., and Tamirat, H., 2019. Medicinal, Nutritional and Anti- Nutritional Properties of Cassava (Manihot esculenta). Academic Journal of Nutrition. 8 (3): 34-46.
[19] Westby, A. 2002. Cassava utilization, storage and small-scale processing. Cassava: Biology, production and utilization, 281-300.
[20] Zakaria, Z. A., Khairi, H. M., Somchit M. N., Sulaiman M. R. 2006. The in vitro Antibacterial Activity and Brine Shrimp Toxicity of Manihot esculenta var. Sri Pontian (Euphorbiacea) Extracts. International Journal of Pharmacology, 2 (2): 216-220.
[21] Zenon, M., Lenilson S. Genelane C. Francine F., Marcelo C. 2017. Effect of Tamarindus indica L. And Manihot esculenta Extracts on Antibiotic-resistant Bacteria. Pharmacognosy Res, 9 (2): 195–199.
Cite This Article
  • APA Style

    Fekadu Zema Mena, Yeshanew Fikre Ololo. (2022). Identification Phytochemicals and Antibacterial Activity of Coir Pith Part of Cassava Root. American Journal of BioScience, 10(5), 180-185. https://doi.org/10.11648/j.ajbio.20221005.14

    Copy | Download

    ACS Style

    Fekadu Zema Mena; Yeshanew Fikre Ololo. Identification Phytochemicals and Antibacterial Activity of Coir Pith Part of Cassava Root. Am. J. BioScience 2022, 10(5), 180-185. doi: 10.11648/j.ajbio.20221005.14

    Copy | Download

    AMA Style

    Fekadu Zema Mena, Yeshanew Fikre Ololo. Identification Phytochemicals and Antibacterial Activity of Coir Pith Part of Cassava Root. Am J BioScience. 2022;10(5):180-185. doi: 10.11648/j.ajbio.20221005.14

    Copy | Download

  • @article{10.11648/j.ajbio.20221005.14,
      author = {Fekadu Zema Mena and Yeshanew Fikre Ololo},
      title = {Identification Phytochemicals and Antibacterial Activity of Coir Pith Part of Cassava Root},
      journal = {American Journal of BioScience},
      volume = {10},
      number = {5},
      pages = {180-185},
      doi = {10.11648/j.ajbio.20221005.14},
      url = {https://doi.org/10.11648/j.ajbio.20221005.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20221005.14},
      abstract = {The purpose of the present study was to identify phytochemicals and anti-microbial activity of the coir pith part extract of cassava root. Preliminary Phytochemical screening was carried out for all the three extracts: n-hexane, methanol and ethanol as per the standard methods. Antibacterial activity of methanol crude extract of the coir pith part (M. esculenta) was performed and it was carried out by using agar well diffusion method. The preliminary phytochemical screening shows alkaloids and flavonoids present only on methanol crude extract and tannins, saponins, flavonoids, glycosides steroids, terpenoids and phenols are available on ethanol, methanol and n- hexane crude extracts. The zone of inhibition of methanolic extract of M. esculenta is 13.67 ± 0.58 mm, 16.67 ± 0.57mm and 18.33 ± 0.58mm at concentration of 25mg/ml, 50mg/ml and 100mg/ml in P. aeruginosa, respectively, and 12.33 ± 1.53mm, 13 ± 1.00mm and 15.33 ± 0.58mm at concentration of 25mg/ml, 50mg/ml and 100mg/ml in S. aureus, respectively. Moreover, the extract also showed moderate antibacterial activity at all concentrations in the range between 12 mm to 18mm on both tested organisms. So, the present study concludes that the coir pith extract anti-nutrients because of the presence of tannins, saponins, steroids and phenols.},
     year = {2022}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Identification Phytochemicals and Antibacterial Activity of Coir Pith Part of Cassava Root
    AU  - Fekadu Zema Mena
    AU  - Yeshanew Fikre Ololo
    Y1  - 2022/10/30
    PY  - 2022
    N1  - https://doi.org/10.11648/j.ajbio.20221005.14
    DO  - 10.11648/j.ajbio.20221005.14
    T2  - American Journal of BioScience
    JF  - American Journal of BioScience
    JO  - American Journal of BioScience
    SP  - 180
    EP  - 185
    PB  - Science Publishing Group
    SN  - 2330-0167
    UR  - https://doi.org/10.11648/j.ajbio.20221005.14
    AB  - The purpose of the present study was to identify phytochemicals and anti-microbial activity of the coir pith part extract of cassava root. Preliminary Phytochemical screening was carried out for all the three extracts: n-hexane, methanol and ethanol as per the standard methods. Antibacterial activity of methanol crude extract of the coir pith part (M. esculenta) was performed and it was carried out by using agar well diffusion method. The preliminary phytochemical screening shows alkaloids and flavonoids present only on methanol crude extract and tannins, saponins, flavonoids, glycosides steroids, terpenoids and phenols are available on ethanol, methanol and n- hexane crude extracts. The zone of inhibition of methanolic extract of M. esculenta is 13.67 ± 0.58 mm, 16.67 ± 0.57mm and 18.33 ± 0.58mm at concentration of 25mg/ml, 50mg/ml and 100mg/ml in P. aeruginosa, respectively, and 12.33 ± 1.53mm, 13 ± 1.00mm and 15.33 ± 0.58mm at concentration of 25mg/ml, 50mg/ml and 100mg/ml in S. aureus, respectively. Moreover, the extract also showed moderate antibacterial activity at all concentrations in the range between 12 mm to 18mm on both tested organisms. So, the present study concludes that the coir pith extract anti-nutrients because of the presence of tannins, saponins, steroids and phenols.
    VL  - 10
    IS  - 5
    ER  - 

    Copy | Download

Author Information
  • Department of Physics, College of Natural and Computational Sciences, Wolaita Sodo University, Wolaita, Ethiopia

  • Department of Physics, College of Natural and Computational Sciences, Wolaita Sodo University, Wolaita, Ethiopia

  • Sections