| Peer-Reviewed

Anti-Helicobacter pylori Activity of Biosynthesized Silver Nanoparticles

Received: 3 June 2022     Accepted: 19 August 2022     Published: 31 August 2022
Views:       Downloads:
Abstract

Background: Recently, there was a great demand for a new, safe, natural, ecofriendly and low cost production of nano-sized silver based antibiotics which become increasingly important in order to resolve the difficulties related with antimicrobial functionalities. Objective: The present study investigates the antibacterial activity of the biosynthesized silver nanoparticles from the hot aqueous extract of the red cabbage Brassica oleracea var capitata f. rubra against the Helicobacter pylori after isolating and characterizing it by microbiological and biochemical tests. Method: The silver nanoparticles have been characterized by using the ultra Violet Visible (UV-vis), X-Ray Diffraction (XRD), the Atomic Force Microscope (AFM) and by scanning Electron Microscope (SEM) coupled with Energy Dispersive X-Ray (EDX). The biosynthesized silver nanoparticles from red cabbage were tested for their antimicrobial activity by 96-wells micro titter plate method against pathogenic H. pylori that clinically isolated from biopsies of patient with stomach ulcers. Result: It was found that the silver nanoparticles have spherical shape with a mean size of 42.34 nm. The study also reveals that the silver nanoparticles have an efficient inhibitory on the bacteria. It was easy to synthesize the silver nanoparticles by this eco-friendly biological method. Conclusion: The biosynthesized silver nanoparticles were efficient to be used as natural antibiotic to treat the stomach ulcer.

Published in American Journal of BioScience (Volume 10, Issue 4)
DOI 10.11648/j.ajbio.20221004.15
Page(s) 153-159
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Silver Nanoparticles, Helicobacter pylori, Antibacterial Activity of Silver Nanoparticles, Red Cabbage

References
[1] Atiyah A. A., A. J. Haider and R. M. Dhahi, "Cytotoxicity properties of functionalised carbon nanotubes on pathogenic bacteria," in IET Nanobiotechnology, vol. 13, no. 6, pp. 597-601, 8 2019, doi: 10.1049/iet-nbt.2018.5394.
[2] Sivakumar, P.; Nethra Devi, C. & Renganathan, S. Synthesis of silver nanoparticles using Lantana camara fruit extract and its effect on pathogens. Asian Journal. Pharm. Clin. Res., 2012, 5 (3): 97-101.
[3] Farooqui, M. D. A.; Chauhan, P. S.; Krishnamoorthy, P. & Shaik, J. Extraction of silver Nanoparticle from the leaf extracts of Clerodendrum inerme, Dig. Journal Nanomat. Biostruct, 2010, 543-549.
[4] Parsonnet J. Bacterial infection as a cause of cancer Environ Health Perspect, 103 Suppl, 19958: 263-268.
[5] Garcia A, Salas-Jara MJ, Herrera C, & Gonzlez, C. Biofilm and Helicobacter pylori: from environment to human host. World Journal Gastroenterol, 2014, 20: 5632-5638.
[6] Matlob. A. N., E. S. Mohammad & K. S. Adui. Production of Vegetables, Part one National library printing and publishing Directorate, University of Mosul, Ministry of Higher Education and Scientific Research. Iraq. 1989, pp. 373.
[7] Tamileswari, R., Nisha, M. H., & Jesurani, S. S. Green (Cauliflower) and Brassica Oleracea Capitata (Cabbage) and the Analysis of Antimicrobial Activity. International Journal of Engineering and Technical Research, 2015, 4: 1071-1074.
[8] Shashi Prabha Dubey, Manu Lahtinen & Mika Sllanpaa. Green synthesis and characterization of silver and gold nanoparticles using leaf extract of Rose rugosa. Physicochemical and Engineering Aspects, 2010, 364 (1-3): 34-41.
[9] Bragg. The structure of some crystals as indicated by their diffraction of X_rays. Proc R Soc Lond 1913, A89 (610): 248-277.
[10] Doaa, K. M. Synthesis and characterization of silver nanoparticles and their antibacterial activity in vitro. M.Sc. Thesis Institute of Genetic Engineering and Biotechnology for Post Graduate Studies, 2015, University of Baghdad. Baghdad. Iraq.
[11] Radeef H. M. A comparison between spiral and spherical shapes in H. pylori isolated from people suffereing from duodenal ulcer. 2016, Thesis, College of Science, Baghdad, Iraq.
[12] Al-Sulami, A. Al-Kiat, H. S. Bakker, L, K. & Hunoon, H. Primary isolation and detection of Helicobacter pylori from dyspeptic patients a simple, rapid method. La Revue de sante dela Mediterrane Orientale. 2018, 14 (2): 268-276.
[13] Parsonnet, jan., Weleh, K., Compton, C., Strauss, R., Timothy, W., Kelsey, P, & Ferraro, M. J. Simple microbiological detection of Campylobacter pylori. Journal. Clin. microbial. 1998, 926 (5); 948-949.
[14] Forbes, B. A., Sahm, D. F., Weissfeld, A. S., & Bailey, S. S. Diagnostic microbiology 12th Edition: Mosby Elsevier, St. Louis, MO, 2007, 778-781.
[15] Toole G. A & Kolter R. Initiation of biofilm formation in pseudomonas fluorescents WCS465 proceeds via multiple, convergent signalling pathways; agenetic analysis. Mol, Microbiol, 1998, (28). 449-461.
[16] Kasi Gopinath, Shanmugam Gowri & Ayyakannu Arumugam. Photosynthesis of silver nanoparticles using Pterocarpus Santalinus leaf extract and their antibacterial properties. Journal of Nanostructure in Chemistry 2013. 3: 68.
[17] Sun, Y. P., Atorngitjawat, P. & Meziani, M. J. Preparation of Silver Nanoparticles Via Rapid Expansion of Water in carbon dioxide microemulsion into reduction solution, Langmuir, 2001, 17: 5707-5710.
[18] Amal Kumar Mondal, Sanjukta Mondal (parui),. Sumana Samanta and Sudebi Mallick. Synthesis of Ecofriendly Silver Nanoparticles from Plant Latex used as an Important Taxonomic Tool for Phylogenetic Inter relationship. Advances in Bioresearch, 2011, 2 (1): 122-133.
[19] Nadia A. Soliman, Eeman H. Ismail, Heba I. Abd EI-Moaty, D. Y. Sabry, Mustafa M. H. Khalil. Anti-Helicobacter pylori, Anti-Diabetic and Cytotoxicity Activity of Biosynthesized Gold Nanoparticles Using Moricandia Nitens Water. Journal. chem. 2018, 61, (4), 691-703.
[20] Morones, J. R., Elechiguerra, J. L., Camacho, A., Holt, K., Kouri, J. B., Ramirez, J. T., Yacaman, M. J. The bactericidal effect of silver nanoparticles. Nanotechnology, 2005, 16 (10): 2346-2353.
Cite This Article
  • APA Style

    Randa Mohammed Dhahi, Bushra Shehab Hamad. (2022). Anti-Helicobacter pylori Activity of Biosynthesized Silver Nanoparticles. American Journal of BioScience, 10(4), 153-159. https://doi.org/10.11648/j.ajbio.20221004.15

    Copy | Download

    ACS Style

    Randa Mohammed Dhahi; Bushra Shehab Hamad. Anti-Helicobacter pylori Activity of Biosynthesized Silver Nanoparticles. Am. J. BioScience 2022, 10(4), 153-159. doi: 10.11648/j.ajbio.20221004.15

    Copy | Download

    AMA Style

    Randa Mohammed Dhahi, Bushra Shehab Hamad. Anti-Helicobacter pylori Activity of Biosynthesized Silver Nanoparticles. Am J BioScience. 2022;10(4):153-159. doi: 10.11648/j.ajbio.20221004.15

    Copy | Download

  • @article{10.11648/j.ajbio.20221004.15,
      author = {Randa Mohammed Dhahi and Bushra Shehab Hamad},
      title = {Anti-Helicobacter pylori Activity of Biosynthesized Silver Nanoparticles},
      journal = {American Journal of BioScience},
      volume = {10},
      number = {4},
      pages = {153-159},
      doi = {10.11648/j.ajbio.20221004.15},
      url = {https://doi.org/10.11648/j.ajbio.20221004.15},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20221004.15},
      abstract = {Background: Recently, there was a great demand for a new, safe, natural, ecofriendly and low cost production of nano-sized silver based antibiotics which become increasingly important in order to resolve the difficulties related with antimicrobial functionalities. Objective: The present study investigates the antibacterial activity of the biosynthesized silver nanoparticles from the hot aqueous extract of the red cabbage Brassica oleracea var capitata f. rubra against the Helicobacter pylori after isolating and characterizing it by microbiological and biochemical tests. Method: The silver nanoparticles have been characterized by using the ultra Violet Visible (UV-vis), X-Ray Diffraction (XRD), the Atomic Force Microscope (AFM) and by scanning Electron Microscope (SEM) coupled with Energy Dispersive X-Ray (EDX). The biosynthesized silver nanoparticles from red cabbage were tested for their antimicrobial activity by 96-wells micro titter plate method against pathogenic H. pylori that clinically isolated from biopsies of patient with stomach ulcers. Result: It was found that the silver nanoparticles have spherical shape with a mean size of 42.34 nm. The study also reveals that the silver nanoparticles have an efficient inhibitory on the bacteria. It was easy to synthesize the silver nanoparticles by this eco-friendly biological method. Conclusion: The biosynthesized silver nanoparticles were efficient to be used as natural antibiotic to treat the stomach ulcer.},
     year = {2022}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Anti-Helicobacter pylori Activity of Biosynthesized Silver Nanoparticles
    AU  - Randa Mohammed Dhahi
    AU  - Bushra Shehab Hamad
    Y1  - 2022/08/31
    PY  - 2022
    N1  - https://doi.org/10.11648/j.ajbio.20221004.15
    DO  - 10.11648/j.ajbio.20221004.15
    T2  - American Journal of BioScience
    JF  - American Journal of BioScience
    JO  - American Journal of BioScience
    SP  - 153
    EP  - 159
    PB  - Science Publishing Group
    SN  - 2330-0167
    UR  - https://doi.org/10.11648/j.ajbio.20221004.15
    AB  - Background: Recently, there was a great demand for a new, safe, natural, ecofriendly and low cost production of nano-sized silver based antibiotics which become increasingly important in order to resolve the difficulties related with antimicrobial functionalities. Objective: The present study investigates the antibacterial activity of the biosynthesized silver nanoparticles from the hot aqueous extract of the red cabbage Brassica oleracea var capitata f. rubra against the Helicobacter pylori after isolating and characterizing it by microbiological and biochemical tests. Method: The silver nanoparticles have been characterized by using the ultra Violet Visible (UV-vis), X-Ray Diffraction (XRD), the Atomic Force Microscope (AFM) and by scanning Electron Microscope (SEM) coupled with Energy Dispersive X-Ray (EDX). The biosynthesized silver nanoparticles from red cabbage were tested for their antimicrobial activity by 96-wells micro titter plate method against pathogenic H. pylori that clinically isolated from biopsies of patient with stomach ulcers. Result: It was found that the silver nanoparticles have spherical shape with a mean size of 42.34 nm. The study also reveals that the silver nanoparticles have an efficient inhibitory on the bacteria. It was easy to synthesize the silver nanoparticles by this eco-friendly biological method. Conclusion: The biosynthesized silver nanoparticles were efficient to be used as natural antibiotic to treat the stomach ulcer.
    VL  - 10
    IS  - 4
    ER  - 

    Copy | Download

Author Information
  • Biology Department, Al-Iraqia University, Baghdad, Iraq

  • Biology Department, Al-Iraqia University, Baghdad, Iraq

  • Sections